A GMC Oxidoreductase Homologue Is Required for Acetylation of Glycopeptidolipid in Mycobacterium smegmatis

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• 作者：Jin Gao ; Nicole S. Sampson
• 刊名：Biochemistry
• 出版年：2014
• 出版时间：February 4, 2014
• 年：2014
• 卷：53
• 期：4
• 页码：611-613
• 全文大小：192K
• ISSN：1520-4995

文摘

The Mycobacterium tuberculosis Rv3409c gene is required for modulation of the Toll-like receptor 2 (TLR-2) signaling response in infected macrophages. Although each is annotated as encoding a cholesterol oxidase, neither Rv3409c nor its ortholog MSMEG1604 is required for the metabolism of cholesterol in mycobacteria. Here we report that a unique lipid, L1334, accumulates in a MSMEG1604 transposon mutant in the Mycobacterium smegmatis cell envelope. L1334 is a polar glycopeptidolipid that is hyperrhamnosylated and in which the 6-deoxytalose moiety is not acetylated. The alteration of L1334 acetylation is consistent with a reduced level of interference with TLR-2 signaling in mutant infected macrophages.