Immunochemical Screening of Pesticides (Simazine and Cypermethrin) in Orange Oil

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• 作者：Mikaela Nichkova ; Xun Fu ; Zheng Yang ; Ping Zhong ; James R. Sanborn ; Dan Chang ; Shirley J. Gee ; Bruce D. Hammock
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：July 8, 2009
• 年：2009
• 卷：57
• 期：13
• 页码：5673-5679
• 全文大小：731K
• 年卷期：v.57,no.13(July 8, 2009)
• ISSN：1520-5118

文摘

Pesticide residue analysis in citrus oils is very important for their quality and marketing. This study assessed the reliability and sensitivity of enzyme-linked immunosorbent assays (ELISA) for simazine and cypermethrin screening in orange oil. Simazine was analyzed after extraction of the oil with methanolic phosphate buffer with a limit of quantitation (LOQ) of 40 μg/L for 1-fold and 100 μg/L for 10-fold oils. Due to matrix effects the immunoanalysis of cypermethrin required hexane−acetonitrile partitioning followed by silica solid phase extraction. The method detected levels higher than 0.5 ppm (mg/L). This LOQ is lower than the U.S. EPA tolerance level (0.9 ppm) for cypermethrin in citrus oils. A good correlation (r² 0.99) between ELISA and LC-MS/MS was observed for the analysis of both analytes in 1-fold orange oil. Immunochemical screening can be used to reduce instrumental analysis costs by its use in preliminary orange oil screening.