Protein-Mediated Efficient Synergistic 鈥淎ntenna Effect鈥?in a Ternary System in D2O Medium

详细信息    查看全文

• 作者：Shyamal Kr Ghorai ; Swarna Kamal Samanta ; Manini Mukherjee ; Sanjib Ghosh
• 刊名：The Journal of Physical Chemistry A
• 出版年：2012
• 出版时间：August 16, 2012
• 年：2012
• 卷：116
• 期：32
• 页码：8303-8312
• 全文大小：476K
• 年卷期：v.116,no.32(August 16, 2012)
• ISSN：1520-5215

文摘

A ternary system consisting of a protein, catechin (either + or 鈥?epimer), and Tb(III) in suitable aqueous buffer medium at physiological pH (= 6.8) has been shown to exhibit highly efficient 鈥渁ntenna effect鈥? Steady state and time-resolved emission studies of each component in the binary complexes (protein with Tb(III) and (+)- or (鈭?-catechin with Tb(III)) and the ternary systems along with the molecular docking studies reveal that the efficient sensitization could be ascribed to the effective shielding of microenvironment of Tb(III) from O鈥揌 oscillator and increased Tb鈥揅 (+/鈭? interaction in the ternary systems in aqueous medium. The ternary system exhibits protein-mediated efficient antenna effect in D₂O medium due to synergistic ET from both the lowest 蟺蟺* triplet state of Trp residue in protein and that of catechin apart from protection of the Tb(III) environment from matrix vibration. The simple system consisting of (+)- or (鈭?-catechin and Tb(III) in D₂O buffer at pH 6.8 has been prescribed to be a useful biosensor.