Detection of Reactive Oxygen Species by a Carbon-Dot–Ascorbic Acid Hydrogel

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• 作者：Sagarika Bhattacharya ; Rhitajit Sarkar ; Sukhendu Nandi ; Angel Porgador ; Raz Jelinek
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：January 3, 2017
• 年：2017
• 卷：89
• 期：1
• 页码：830-836
• 全文大小：426K
• ISSN：1520-6882

文摘

Detection of reactive oxygen species (ROS) is important in varied biological processes, disease diagnostics, and chemotherapeutic drug screening. We constructed a ROS sensor comprising an ascorbic-acid-based hydrogel encapsulating luminescent amphiphilic carbon-dots (C-dots). The sensing mechanism is based upon ROS-induced oxidation of the ascorbic acid units within the hydrogel scaffold; as a consequence, the hydrogel framework collapses resulting in aggregation of the C-dots and quenching of their luminescence. The C-dot–hydrogel platform exhibits high sensitivity and detected ROS generated chemically in solution and in actual cell environments. We demonstrate application of the C-dot–hydrogel for evaluating the efficacy of a chemotherapeutic substance, underscoring the potential of the system for drug screening applications.