New Nucleotide-Competitive Non-Nucleoside Inhibitors of Terminal Deoxynucleotidyl Transferase: Discovery, Characterization, and Crystal Structure in Complex with the Target

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• 作者：Roberta Costi ; Giuliana Cuzzucoli Crucitti ; Luca Pescatori ; Antonella Messore ; Luigi Scipione ; Silvano Tortorella ; Alessandra Amoroso ; Emmanuele Crespan ; Pietro Campiglia ; Bruno Maresca ; Amalia Porta ; Ilaria Granata ; Ettore Novellino ; J茅r么me Gouge ; Marc Delarue ; Giovanni Maga ; Roberto Di Santo
• 刊名：Journal of Medicinal Chemistry
• 出版年：2013
• 出版时间：September 26, 2013
• 年：2013
• 卷：56
• 期：18
• 页码：7431-7441
• 全文大小：484K
• 年卷期：v.56,no.18(September 26, 2013)
• ISSN：1520-4804

文摘

Terminal deoxynucletidyl transferase (TdT) is overexpressed in some cancer types, where it might compete with pol 渭 during the mutagenic repair of double strand breaks (DSBs) through the nonhomologous end joining (NHEJ) pathway. Here we report the discovery and characterization of pyrrolyl and indolyl diketo acids that specifically target TdT and behave as nucleotide-competitive inhibitors. These compounds show a selective toxicity toward MOLT-4 compared to HeLa cells that correlate well with in vitro selectivity for TdT. The binding site of two of these inhibitors was determined by cocrystallization with TdT, explaining why these compounds are competitive inhibitors of the deoxynucleotide triphosphate (dNTP). In addition, because of the observed dual localization of the phenyl substituent, these studies open the possibility of rationally designing more potent compounds.