Application of Spiroplasma melliferum Proteogenomic Profiling for the Discovery of Virulence Factors and Pathogenicity Mechanisms in Host-associated Spiroplasmas
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- 作者：Dmitry Alexeev ; Elena Kostrjukova ; Alexander Aliper ; Anna Popenko ; Nikolay Bazaleev ; Alexander Tyakht ; Oksana Selezneva ; Tatyana Akopian ; Elena Prichodko ; Ilya Kondratov ; Mikhail Chukin ; Irina Demina ; Maria Galyamina ; Dmitri Kamashev ; Anna Vanyushkina ; Valentina Ladygina ; Sergei Levitskii ; Vasily Lazarev ; Vadim Govorun
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：January 1, 2012
- 年：2012
- 卷：11
- 期：1
- 页码：224-236
- 全文大小：378K
- 年卷期：v.11,no.1(January 1, 2012)
- ISSN：1535-3907
文摘
To date, no genome of any of the species from the genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current genome sequencing technologies. Here, we report the assembly of the Spiroplasma melliferum KC3 genome into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on the discovery of 521 expressed proteins and comprehensive metabolomic profiling. A systems approach allowed us to elucidate putative pathogenicity mechanisms and to discover major virulence factors, such as Chitinase utilization enzymes and toxins never before reported for insect pathogenic spiroplasmas.