Immunochromatography Using Colloidal Gold-Antibody Probe for the Detection of Atrazine in Water Samples
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- 作者：Won-Bo Shim ; Zheng-You Yang ; Ji-Young Kim ; Jin-Gil Choi ; Jung-Hyun Je ; Sung-Jo Kang ; Anna Yu Kolosova ; Sergei A. Eremin ; Duck-Hwa Chung
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：December 27, 2006
- 年：2006
- 卷：54
- 期：26
- 页码：9728 - 9734
- 全文大小：192K
- 年卷期：v.54,no.26(December 27, 2006)
- ISSN：1520-5118
文摘
An immunochromatography (ICG) strip test for rapid detection of atrazine in water samples wasdeveloped. A monoclonal antibody (MAb) specific to atrazine was produced from the cloned hybridomacell (AT-1-M3) and used to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) and ICG strip. MAb conjugated to colloidal gold, and that was applied to the conjugate padof the ICG strip. The visual detection limit for the ICG strip was 3 ng/mL. This test required only 10min to get results and one step of sample to perform the assay. The results of water samples spikedwith 5, 10, 20, and 50 ng/mL of atrazine by ICG strip were in good agreement with those obtainedby DC-ELISA. The ICG strip was sufficiently sensitive and accurate to be useful for rapid screeningof atrazine in various water samples.