Characterization and Application of BiLA, a Psychrophilic α-Amylase from Bifidobacterium longum
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- 作者：Hye-Won Lee ; Hye-Yeon Jeon ; Hye-Jeong Choi ; Na-Ri Kim ; Woo-Jae Choung ; Ye-Seul Koo ; Dam-Seul Ko ; SangGuan You ; Jae-Hoon Shim
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2016
- 出版时间：April 6, 2016
- 年：2016
- 卷：64
- 期：13
- 页码：2709-2718
- 全文大小：644K
- ISSN：1520-5118
文摘
In this study, a novel α-amylase was cloned from Bifidobacterium longum and named BiLA. The enzyme exhibited optimal activity at 20 °C and a pH value of 5.0. Kinetic analysis using various carbohydrate substrates revealed that BiLA had the highest kcat/Km value for amylose. Interestingly, analysis of the enzymatic reaction products demonstrated that BiLA specifically catalyzed the hydrolysis of oligosaccharides and starches up to G5 from the nonreducing ends. To determine whether BiLA can be used to generate slowly digestible starch (SDS), starch was treated with BiLA, and the kinetic parameters were analyzed using porcine pancreatic α-amylase (PPA) and amyloglucosidase (AMG). Compared to normal starch, BiLA-treated starch showed lower kcat/Km values with PPA and AMG, suggesting that BiLA is a potential candidate for the production of SDS.