Elastin-Like Polypeptide Based Hydroxyapatite Bionanocomposites

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• 作者：Eddie Wang ; Sang-Hyuk Lee ; Seung-Wuk Lee
• 刊名：Biomacromolecules
• 出版年：2011
• 出版时间：March 14, 2011
• 年：2011
• 卷：12
• 期：3
• 页码：672-680
• 全文大小：1094K
• 年卷期：v.12,no.3(March 14, 2011)
• ISSN：1526-4602

文摘

In nature, organic matrix macromolecules play a critical role in enhancing the mechanical properties of biomineralized composites such as bone and teeth. Designing artificial matrix analogues is promising but challenging because relatively little is known about how natural matrix components function. Therefore, in lieu of using natural components, we created biomimetic matrices using genetically engineered elastin-like polypeptides (ELPs) and then used them to construct mechanically robust ELP-hydroxyapatite (HAP) composites. ELPs were engineered with well-defined backbone charge distributions by periodic incorporation of negative, positive, or neutral side chains or with HAP-binding octaglutamic acid motifs at one or both protein termini. ELPs exhibited sequence-specific capacities to interact with ions, bind HAP, and disperse HAP nanoparticles. HAP-binding ELPs were incorporated into calcium phosphate cements, resulting in materials with improved mechanical strength, injectability, and antiwashout properties. The results demonstrate that rational design of genetically engineered polymers is a powerful system for determining sequence鈭抪roperty relationships and for improving the properties of organic鈭抜norganic composites. Our approach may be used to further develop novel, multifunctional bone cements and expanded to the design of other advanced composites.