Development of Marker-Free Transgenic Potato Tubers Enriched in Caffeoylquinic Acids and Flavonols

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• 作者：Yang Li ; Wenzhao Tang ; Jing Chen ; Ru Jia ; Lianjie Ma ; Shaoli Wang ; Jiao Wang ; Xiangling Shen ; Zhaohui Chu ; Changxiang Zhu ; Xinhua Ding
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2016
• 出版时间：April 13, 2016
• 年：2016
• 卷：64
• 期：14
• 页码：2932-2940
• 全文大小：522K
• 年卷期：0
• ISSN：1520-5118

文摘

Potato (Solanum tuberosum L.) is a major crop worldwide that meets human economic and nutritional requirements. Potato has several advantages over other crops: easy to cultivate and store, cheap to consume, and rich in a variety of secondary metabolites. In this study, we generated three marker-free transgenic potato lines that expressed the Arabidopsis thaliana flavonol-specific transcriptional activator AtMYB12 driven by the tuber-specific promoter Patatin. Marker-free potato tubers displayed increased amounts of caffeoylquinic acids (CQAs) (3.35-fold increases on average) and flavonols (4.50-fold increase on average). Concentrations of these metabolites were associated with the enhanced expression of genes in the CQA and flavonol biosynthesis pathways. Accumulation of CQAs and flavonols resulted in 2-fold higher antioxidant capacity compared to wild-type potatoes. Tubers from these marker-free transgenic potatoes have therefore improved antioxidant properties.