Trichohyalin is a Potential Major Autoantigen in Human Alopecia Areata
详细信息 查看全文
- 作者：Man Ching Leung ; Chris W. Sutton ; David A. Fenton ; Desmond J. Tobin
- 刊名：Journal of Proteome Research
- 出版年：2010
- 出版时间：October 1, 2010
- 年：2010
- 卷：9
- 期：10
- 页码：5153-5163
- 全文大小：397K
- 年卷期：v.9,no.10(October 1, 2010)
- ISSN：1535-3907
文摘
Several lines of evidence support an autoimmune basis for alopecia areata (AA), a common putative autoimmune hair loss disorder. However, definitive support is lacking largely because the identity of hair follicle (HF) autoantigen(s) involved in its pathogenesis remains unknown. Here, we isolated AA-reactive HF-specific antigens from normal human scalp anagen HF extracts by immunoprecipitation using serum antibodies from 10 AA patients. Samples were analyzed by LC−MALDI-TOF/TOF mass spectrometry, which indicated strong reactivity to the hair growth phase-specific structural protein trichohyalin in all AA sera. Keratin 16 (K16) was also identified as another potential AA-relevant target HF antigen. Double immunofluorescence studies using AA (and control sera) together with a monoclonal antibody to trichohyalin revealed that AA sera contained immunoreactivity that colocalized with trichohyalin in the growth phase-specific inner root sheath of HF. Furthermore, a partial colocalization of AA serum reactivity with anti-K16 antibody was observed in the outer root sheath of the HF. In summary, this study supports the involvement of an immune response to anagen-specific HFs antigens in AA and specifically suggests that an immune response to trichohyalin and K16 may have a role in the pathogenesis of the enigmatic disorder.