The Immobilization of Glucose Oxidase at Manganese Dioxide Particles-Decorated Reduced Graphene Oxide Sheets for the Fabrication of a Glucose Biosensor

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• 作者：A. T. Ezhil Vilian ; Veerappan Mani ; Shen-Ming Chen ; Bose Dinesh ; Sheng-Tung Huang
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2014
• 出版时间：October 8, 2014
• 年：2014
• 卷：53
• 期：40
• 页码：15582-15589
• 全文大小：453K
• ISSN：1520-5045

文摘

Here, we describe a simple approach for the efficient immobilization of poly-l-lysine (PLL) impregnated glucose oxidase (GOx) at a manganese dioxide (MnO₂)-decorated chemically reduced graphene oxide (CRGO) composite film modified electrode. The MnO₂/CRGO film was analyzed by various techniques such as scanning electron microscopy, UV鈥搗isible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and electrochemical impedance spectroscopy. MnO₂ particles are uniformly deposited at the CRGO surface through an easily adoptable electropolymerization method. The as-prepared (GOx-PLL/MnO₂/CRGO) composite film exhibited a surface coverage concentration (螕) of 2.68 脳 10^鈥?0 mol/cm^鈥?, indicative of high GOx loading. The heterogeneous rate constant for the redox reaction of GOx has been calculated to be 4.92 s^鈥?. A glucose biosensor was fabricated which exhibits a wide linear range from 0.04 to 10 mM with a limit of detection of 0.02 mM (S/N = 3). The proposed inexpensive, simple, and sensitive sensing approach based on the MnO₂/CRGO nanocomposite electrode can be a promising method for the determination of glucose present in the clinical and environmental food analysis.