A Unique Flavin Mononucleotide-Linked Primary Alcohol Oxidase for Glycopeptide A40926 Maturation
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- 作者：Yi-Shan Li ; Jin-Yuan Ho ; Chia-Chi Huang ; Syue-Yi Lyu ; Chun-Yen Lee ; Yu-Ting Huang ; Chang-Jer Wu ; Hsiu-Chien Chan ; Chuan-Jiuan Huang ; Ning-Shian Hsu ; Ming-Daw Tsai ; Tsung-Lin Li
- 刊名：Journal of the American Chemical Society
- 出版年：2007
- 出版时间：November 7, 2007
- 年：2007
- 卷：129
- 期：44
- 页码：13384 - 13385
- 全文大小：490K
- 年卷期：v.129,no.44(November 7, 2007)
- ISSN：1520-5126
文摘
The unique pharmacokinetic and pharmacodynamic activities of glycopeptide antibiotics are conferred by tailoring steps occurring on the aglycone. Here, we report that protein Dbv29, involved in the biosynthesis of A40926, is a novel flavin mononucleotide-dependent primary alcohol glycopeptide hexose oxidase that carries out a four-electron oxidation reaction. Dbv29 catalyzes the last step in a multistep sequence to complete N-acyl aminoglucuronic acid substituent biosynthesis for a potent drug lead. The characterized enzyme may provide a new way to enhance the efficacy of currently used glycopeptide drugs. This detailed function-mechanism analysis of the enzyme increases our knowledge of this new class of enzyme.