Protein Engineering of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase into Parkeol Synthase
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- 作者：Yuan-Ting Liu ; Tain-Chang Hu ; Cheng-Hsiang Chang ; Wen-Shiang Shie ; Tung-Kung Wu
- 刊名：Organic Letters
- 出版年：2012
- 出版时间：October 19, 2012
- 年：2012
- 卷：14
- 期：20
- 页码：5222-5225
- 全文大小：226K
- 年卷期：v.14,no.20(October 19, 2012)
- ISSN：1523-7052
文摘
A Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase mutant, ERG7T384Y/Q450H/V454I, produced parkeol but not lanosterol as the sole end product. Parkeol undergoes downstream metabolism to generate compounds 9 and 10. In vitro incubation of parkeol produced a product profile similar to that of the in vivo experiment. In summary, parkeol undergoes a metabolic pathway similar to that of cycloartenol in yeast but distinct from that of lanosterol in yeast, suggesting that two different metabolic pathways of postoxidosqualene cyclization may exist in S. cerevisiae.