Formation of Well-Oriented Microtubules with Preferential Polarity in a Confined Space under a Temperature Gradient

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• 作者：Akira Kakugo ; Yoshiki Tamura ; Kazuhiro Shikinaka ; Momoko Yoshida ; Ryuzo Kawamura ; Hidemitsu Furukawa ; Yoshihito Osada ; Jian Ping Gong
• 刊名：Journal of the American Chemical Society
• 出版年：2009
• 出版时间：December 23, 2009
• 年：2009
• 卷：131
• 期：50
• 页码：18089-18095
• 全文大小：420K
• 年卷期：v.131,no.50(December 23, 2009)
• ISSN：1520-5126

文摘

Tubulin polymerization in a confined space under a temperature gradient produced well-oriented microtubule assemblies with preferential polarity. We analyzed the structure and polarity of these assemblies at various levels of resolution by performing polarized light microscopy (millimeter order), fluorescence microscopy (micrometer order), and transmission electron microscopy (nanometer order).