Exchange of Histidine Spacing between Sp1 and GLI Zinc Fingers: Distinct Effect of Histidine Spacing-Linker Region on DNA Binding

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• 作者：Yasuhisa Shiraishi ; Miki Imanishi ; and Yukio Sugiura
• 刊名：Biochemistry
• 出版年：2004
• 出版时间：May 25, 2004
• 年：2004
• 卷：43
• 期：20
• 页码：6352 - 6359
• 全文大小：203K
• 年卷期：v.43,no.20(May 25, 2004)
• ISSN：1520-4995

文摘

In the DNA recognition mode of C₂H₂-type zinc fingers, the finger-finger connection region,consisting of the histidine spacing (HX_3-5H) and linker, would be important for determining the orientationof the zinc finger domains. To clarify the influence of spacing between two ligand histidines in the DNAbinding, we exchanged the histidine spacing between Sp1 and GLI zinc fingers, which have an HX₃H-TGEKK linker (typical) and an HX₄H-SNEKP linker (atypical), respectively. A significant decrease inthe DNA binding affinity and specificity is found in Sp1-type peptides, whereas GLI-type peptides showa mild reduction. To evaluate the effect of the linker characteristics, we further designed Sp1-type mutantswith an SNEKP linker. As a result, the significant effect of the histidine spacing in Sp1-type peptides wasreduced. These results demonstrate that (1) the histidine spacing significantly affects the DNA binding ofzinc finger proteins and (2) the histidine spacing and the following linker regions are one effective targetfor regulating the DNA recognition mode of zinc finger proteins.