Swapping of the -Hairpin Region between Sp1 and GLI Zinc Fingers: Significant Role of the 详细信息    查看全文

• 作者：Yasuhisa Shiraishi ; Miki Imanishi ; Tatsuya Morisaki ; and Yukio Sugiura
• 刊名：Biochemistry
• 出版年：2005
• 出版时间：February 22, 2005
• 年：2005
• 卷：44
• 期：7
• 页码：2523 - 2528
• 全文大小：279K
• 年卷期：v.44,no.7(February 22, 2005)
• ISSN：1520-4995

文摘

The recent design strategy of zinc finger peptides has mainly focused on the -helix region,which plays a direct role in DNA recognition. On the other hand, the study of non-DNA-contacting regionsis extremely scarce. By swapping the -hairpin regions between the Sp1 and GLI zinc fingers, in thisstudy, we investigated how the -hairpin region of the C₂H₂-type zinc finger peptides contributes to theDNA binding properties. Surprisingly, the Sp1 mutant with the GLI-type -hairpin had a higher DNAbinding affinity than that of the wild-type Sp1. The result of the DNase I footprinting analyses also showedthe change in the DNA binding pattern. In contrast, the GLI zinc finger completely lost DNA bindingability as a result of exchanging the -hairpin region. These results strongly indicate that the -hairpinregion appears to function as a scaffold and has an important effect on the DNA binding properties of theC₂H₂-type zinc finger peptides.