Characterization of Acetylcholinesterase Inhibitory Constituents from Annona glabra Assisted by HPLC Microfractionation
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- 作者：Sheng-Fa Tsai ; Shoei-Sheng Lee
- 刊名：Journal of Natural Products
- 出版年：2010
- 出版时间：October 22, 2010
- 年：2010
- 卷：73
- 期：10
- 页码：1632-1635
- 全文大小：177K
- 年卷期：v.73,no.10(October 22, 2010)
- ISSN：1520-6025
文摘
The active fraction of the EtOH extract of the stem of Annona glabra against acetylcholinesterase (AChE) was analyzed by combining HPLC microfractionation with a bioassay. The analytical-scale sample was fractionated by HPLC-DAD into 96-well microplates, which, after evaporation, were assayed against AChE. The active subfractions were scaled up by separation over semipreparative HPLC to give 20 compounds. Four of these, (7S,14S)-(−)-N-methyl-10-O-demethylxylopinine salt (3), S-(−)-7,8-didehydro-10-O-demethylxylopininium salt (10), S-(−)-7,8-didehydrocorydalminium salt (11), and 5-O-methylmarcanine D (17), were assigned as new natural products. In addition, compounds 10 and 11 represent the first natural occurrence of 7,8-didehydroprotoberberines. Compound 3, pseudocolumbamine (12), palmatine (15), and pseudopalmatine (16) showed anti-AChE IC50 values of 8.4, 5.0, 0.4, and 1.8 μM, respectively.