Global Identification of Protein Post-translational Modifications in a Single-Pass Database Search

详细信息    查看全文

• 作者：Michael R. Shortreed ; Craig D. Wenger ; Brian L. Frey ; Gloria M. Sheynkman ; Mark Scalf ; Mark P. Keller ; Alan D. Attie ; Lloyd M. Smith
• 刊名：Journal of Proteome Research
• 出版年：2015
• 出版时间：November 6, 2015
• 年：2015
• 卷：14
• 期：11
• 页码：4714-4720
• 全文大小：405K
• ISSN：1535-3907

文摘

Bottom-up proteomics database search algorithms used for peptide identification cannot comprehensively identify post-translational modifications (PTMs) in a single-pass because of high false discovery rates (FDRs). A new approach to database searching enables global PTM (G-PTM) identification by exclusively looking for curated PTMs, thereby avoiding the FDR penalty experienced during conventional variable modification searches. We identified over 2200 unique, high-confidence modified peptides comprising 26 different PTM types in a single-pass database search.