Adsorption and Covalent Coupling of ATP-Binding DNA Aptamers onto Cellulose

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• 作者：Shunxing Su ; Razvan Nutiu ; Carlos D. M. Filipe ; Yingfu Li ; Robert Pelton
• 刊名：Langmuir
• 出版年：2007
• 出版时间：January 30, 2007
• 年：2007
• 卷：23
• 期：3
• 页码：1300 - 1302
• 全文大小：243K
• 年卷期：v.23,no.3(January 30, 2007)
• ISSN：1520-5827

文摘

With the long-term goal of developing paper surfaces that will detect pathogens, we have investigated physicaladsorption and covalent coupling as strategies for treating cellulose surfaces with a DNA aptamer that binds ATP.Physical adsorption was reversible and the isotherms fitted the Langmuir equation with an adsorption maximum of0.105 mg/m² at high ionic strength (300 mM NaCl, 25 mM Tris-HCl) and only 0.024 mg/m² in lower ionic strengthbuffer (25 mM Tris-HCl). Covalent coupling of amine-terminated aptamer with oxidized cellulose film (Schiff base+ reduction) gave 25% coupling efficiency while maintaining the aptamer activity which was illustrated by usinga known fluorescent aptamer that is capable of ATP detection. Therefore, covalent coupling, without spacer molecules,is a promising approach for supporting biosensing aptamers on cellulose.