Determination of Chloramphenicol Residues in Milk by Enzyme-Linked Immunosorbent Assay: Improvement by Biotin−Streptavidin-Amplified System
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- 作者：Li Wang ; Yan Zhang ; Xiang Gao ; Zhenjuan Duan ; Shuo Wang
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：March 24, 2010
- 年：2010
- 卷：58
- 期：6
- 页码：3265-3270
- 全文大小：781K
- 年卷期：v.58,no.6(March 24, 2010)
- ISSN：1520-5118
文摘
A sensitive biotin−streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA) method was developed for the determination of chloramphenicol residues in milk. The biotin−streptavidin system was applied to enhance the sensitivity. After optimization, the detection limit of the method was found to be 0.042 ± 0.006 ng mL−1, which is 8-fold more sensitive than the traditional competitive ELISA using the same antibody and coating antigen. The amplification mechanism of the biotin−streptavidin system and the major factors affecting the sensitivity of detection are discussed. This method was successfully applied to determine the chloramphenicol residues in milk samples with a simple and rapid extraction procedure, and good recoveries (85.66−109.67%) were obtained. The result indicated that the biotin−streptavidin system may be a valuable tool to improve the specific detection of trace veterinary drug residues and could be widely used for routine monitoring of food samples.