Delphinidin Prevents Muscle Atrophy and Upregulates miR-23a Expression

详细信息    查看全文

• 作者：Motoki Murata ; Haruna Nonaka ; Satomi Komatsu ; Megumi Goto ; Mai Morozumi ; Shuhei Yamada ; I-Chian Lin ; Shuya Yamashita ; Hirofumi Tachibana
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2017
• 出版时间：January 11, 2017
• 年：2017
• 卷：65
• 期：1
• 页码：45-50
• 全文大小：380K
• ISSN：1520-5118

文摘

Delphinidin, one of the major anthocyanidins, shows protective effects against a variety of pathologies, including cancer, inflammation, and muscle atrophy. The purpose of this study was to determine the preventive mechanism of delphinidin on disuse muscle atrophy. In vitro and in vivo models were used to validate the effects of delphinidin on the expression of MuRF1, miR-23a, and NFATc3. Delphinidin suppressed the upregulation of MuRF1 (1.77 ± 0.05 vs 1.03 ± 0.17, P < 0.05) expression and inhibited the downregulation of miR-23a (0.56 ± 0.05 vs 0.94 ± 0.06, P < 0.05) and NFATc3 (0.61 ± 0.02 vs 1.02 ± 0.08, P < 0.01) expression in dexamethasone-treated C2C12 cells. In gastrocnemius, muscle weight loss was prevented by oral administration of delphinidin. Moreover, delphinidin suppressed MuRF1 (3.35 ± 0.13 vs 2.26 ± 0.3, P < 0.01) expression and promoted miR-23a (0.58 ± 0.15 vs 2.25 ± 0.29, P < 0.001) and NFATc3 (0.85 ± 0.17 vs 1.54 ± 0.13, P < 0.001) expressions. Delphinidin intake may prevent disuse muscle atrophy by inducing miR-23a expression and suppressing MuRF1 expression.