Studies on the Chemical Transformation of 20(S)-Protopanaxatriol (PPT)-Type Ginsenosides Re, Rg2, and Rf Using Rapid Resolution Liquid Chromatography Coupled wi
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- 作者：Wei Wu ; Qiujie Qin ; Yingying Guo ; Jinghui Sun ; Shuying Liu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：October 10, 2012
- 年：2012
- 卷：60
- 期：40
- 页码：10007-10014
- 全文大小：343K
- 年卷期：v.60,no.40(October 10, 2012)
- ISSN：1520-5118
文摘
A rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method was developed for analysis of chemical transformation of 20(S)-protopanaxatriol (PPT)-type ginsenosides Re, Rg2, and Rf in acidic conditions. The transformation products were identified by comparing the retention time of the standard compounds, the accurate mass measurement, and the fragment ions obtained from RRLC-Q-TOF-tandem mass spectrometry (MS/MS) analyses. The specific product ions of aglycone PPT (m/z 475), C-24- and C-25-hydrated PPT (m/z 493), and 螖20(21) or 螖20(22) dehydration PPT (m/z 457) by MS/MS were discussed for structural characterization. Experiments demonstrated that chemical transformation mechanisms of 20(S)-PPT-type ginsenosides in acidic conditions include hydrolysis of saccharide substitution, 螖20(21) or 螖20(22) dehydration, and hydration addition reactions at C-24 and C-25. The chemical transformation pathway for 20(S)-PPT-type ginsenosides was summarized. The developed RRLC-Q-TOF-MS method was also applied for comparative analysis of 20(S)-PPT ginsenoside and related chemical transformation products in ginseng products.