Multicolor Imaging of Cancer Cells with Fluorophore-Tagged Aptamers for Single Cell Typing

详细信息    查看全文

• 作者：Song Wang ; Hao Kong ; Xiaoyun Gong ; Sichun Zhang ; Xinrong Zhang
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：August 19, 2014
• 年：2014
• 卷：86
• 期：16
• 页码：8261-8266
• 全文大小：372K
• ISSN：1520-6882

文摘

The discrimination of the type of cancer cells remains challenging due to the subtle differences in their expression of membrane receptors. In this work, we developed a multicolor cell imaging method for distinguishing the type of cancer cells with fluorophore-tagged aptamers. We found that the interaction between aptamers and cancer cells was affected by both of the sequence of aptamers and the labeled dyes. As the co-ownership of biomarkers for different cancer cell lines, the fluorophore-tagged aptamers interacted with different cancer cell lines in different degree, resulting in a distinct color to discriminate the type of cancer cells at single cell level. Taking advantage of the cross-reactive ability of the fluorophore-tagged aptamers, we could not only distinguish the cancerous cells quickly from large quantities of noncancerous cells, but also identify the type of the cancerous cells. This work has potential application for cancer diagnostic and therapy in the future.