Long-Term Fluorescent Cellular Tracing by the Aggregates of AIE Bioconjugates
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- 作者：Zhengke Wang ; Sijie Chen ; Jacky W. Y. Lam ; Wei Qin ; Ryan T. K. Kwok ; Ni Xie ; Qiaoling Hu ; Ben Zhong Tang
- 刊名：The Journal of the American Chemical Society
- 出版年：2013
- 出版时间：June 5, 2013
- 年：2013
- 卷：135
- 期：22
- 页码：8238-8245
- 全文大小：434K
- 年卷期：v.135,no.22(June 5, 2013)
- ISSN：1520-5126
文摘
There is a great demand for long-term cellular tracers because of their great importance in monitoring biological processes, pathological pathways, therapeutic effects, etc. Herein we report a new type of fluorescence 鈥渢urn-on鈥?probe for tracing live cells over a long period of time. We synthesized the fluorogenic probe by attaching a large number of tetraphenylethene (TPE) labels to a chitosan (CS) chain. The resultant TPE鈥揅S bioconjugate shows a unique aggregation-induced emission (AIE) behavior. It is nonfluorescent when dissolved but becomes highly emissive when its molecules are aggregated. The AIE aggregates can be readily internalized by HeLa cells. The cellular staining by the TPE鈥揅S aggregates is so indelible that it enables cell tracing for as long as 15 passages. The internalized AIE aggregates are kept inside the cellular compartments and do not contaminate other cell lines in the coculture systems, permitting the differentiation of specific cancerous cells from normal healthy cells.