Attomole Sensitivity of Staphylococcal Enterotoxin B Detection Using an Aptamer-Modified Surface-Enhanced Raman Scattering Probe

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• 作者：Erhan Temur ; Adem Zengin ; 陌smail Hakk谋 Boyac谋 ; Fahriye Ceyda Dudak ; Hilal Torul ; U臒ur Tamer
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：December 18, 2012
• 年：2012
• 卷：84
• 期：24
• 页码：10600-10606
• 全文大小：398K
• 年卷期：v.84,no.24(December 18, 2012)
• ISSN：1520-6882

文摘

In this report, we present a new homogeneous detection method for staphylococcal enterotoxin B (SEB) utilizing core鈥搒hell-structured iron鈥揼old magnetic nanoparticles and a gold nanorod surface-enhanced Raman scattering (SERS) probe in solution. Peptide ligand (aptamer) functionalized magnetic gold nanorod particles were used as scavengers for target SEB. After the SEB molecules were separated from the matrix, the sandwich assay procedure was tested by gold nanorod particles that act as SERS probes. The binding constant between SEB and peptide鈥搉anoparticle complex was determined as 8.0 脳 10⁷ M^鈥?. The correlation between the SEB concentration and SERS signal was found to be linear within the range of 2.5 fM to 3.2 nM. The limit of detection for the homogeneous assay was determined as 224 aM (ca. 2697 SEB molecules/20 渭L sample volume). Also, gold-coated surfaces were used as capture substrates and performances of the two methods were compared. Furthermore, the developed method was evaluated for investigating the SEB specificity on bovine serum albumin (BSA) and avidin and detecting SEB in artificially contaminated milk, blood, and urine.