Portable Microcoil NMR Detection Coupled to Capillary Electrophoresis

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• 作者：Joana Diekmann ; Kristl L. Adams ; Gregory L. Klunder ; Lee Evans ; Paul Steele ; Carla Vogt ; Julie L. Herberg
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：February 15, 2011
• 年：2011
• 卷：83
• 期：4
• 页码：1328-1335
• 全文大小：883K
• 年卷期：v.83,no.4(February 15, 2011)
• ISSN：1520-6882

文摘

High-efficiency separation techniques, such as capillary electrophoresis (CE), coupled to a nondestructive nuclear magnetic resonance (NMR) spectrometer offer the ability to separate, chemically identify, and provide structural information on analytes in small sample volumes. Previous CE鈭扤MR coupled systems utilized laboratory-scale NMR magnets and spectrometers, which require very long separation capillaries. New technological developments in electronics have reduced the size of the NMR system, and small 1鈭? T permanent magnets provide the possibilities of a truly portable NMR. The microcoils used in portable and laboratory-scale NMR may offer the advantage of improved mass sensitivity because the limit of detection (LOD) is proportional to the coil diameter. In this work, CE is coupled with a portable, briefcase-sized NMR system that incorporates a microcoil probe and a 1.8 T permanent magnet to measure ¹⁹F NMR spectra. Separations of fluorinated molecules are demonstrated with stopped- and continuous-flow NMR detection. The results demonstrate that coupling CE to a portable NMR instrument is feasible and can provide a low-cost method to obtain structural information on microliter samples. An LOD of 31.8 nmol for perfluorotributylamine with a resolution of 4 ppm has been achieved with this system.