Regulation of Multimer Formation in Electrospray Mass Spectrometry

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• 作者：Morgan Stefansson ; Per J. R. Sjö ; berg ; and Karin E. Markides
• 刊名：Analytical Chemistry
• 出版年：1996
• 出版时间：May 15, 1996
• 年：1996
• 卷：68
• 期：10
• 页码：1792 - 1797
• 全文大小：190K
• 年卷期：v.68,no.10(May 15, 1996)
• ISSN：1520-6882

文摘

Extensive multimer formation of small and unchargedanalytes was observed in electrospray mass spectrometry.The concentration-dependent aggregation behavior, including species up to tetramers, resulted in highly nonlinear calibration curves. For some analytes, theseeffectswere operating down into the nanomolar level. Varyingthe drift potential failed to selectively dissociate themultimer complexes in favor of the monomer, whichseverely obstructed quantitative work. However, by incorporating certain cationic additives into the electrosprayed solution, complete suppression of the multimerscould be obtained. In addition, a gain in the signalintensities by a factor up to 7 was achieved. A varietyofadditives were investigated, and the structural requirements for optimum performance will be outlined.