Analysis of Fatty Aldehyde Composition, Including 12-Methyltridecanal, in Plasmalogens from Longissimus Muscle of Concentrate- and Pasture-Fed Bulls
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- 作者：Dirk Dannenberger ; Stephanie Lorenz ; Gerd Nuernberg ; Nigel Scollan ; Klaus Ender ; and Karin Nuernberg
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2006
- 出版时间：January 11, 2006
- 年：2006
- 卷：54
- 期：1
- 页码：182 - 188
- 全文大小：69K
- 年卷期：v.54,no.1(January 11, 2006)
- ISSN：1520-5118
文摘
In a large study, 64 German Holstein and German Simmental bulls were randomly allocated to eitheran indoor concentrate system or periods of pasture feeding followed by a finishing period on aconcentrate containing linseed to enhance the contents of beneficial fatty acids in beef. This paperreports the diet effects on the concentration of 12-methyltridecanal (12-MT) and further fatty aldehydesreleased from plasmalogens in the phospholipids of longissimus muscle of the bulls. Because of thetrace level of the important odorant 12-MT in beef, the determination of fatty aldehydes in phospholipidswas done by acidic hydrolysis and the reaction of the aldehydes with 2,4-DNPH followed by high-pressure liquid chromatography (HPLC) analysis. The diet affected the 12-MT concentrations in themuscle phospholipids of both breeds. Pasture feeding significantly increased the 12-MT concentrationsup to 350 
g/100 g fresh muscle in the muscle phospholipids of German Holstein and GermanSimmental bulls as compared with the concentrate-fed bulls. Furthermore, pasture feeding resultedin a significant increase of n-octadecanal in the muscle phospholipids of both breeds up to 39.5mg/100 g fresh muscle. The concentration of n-hexadecanal was not affected by the diet. Pasturefeeding as compared to concentrate feeding significantly decreased the concentration of n-octadec-9-enal in the muscle phospholipids. Summarizing, pasture feeding increased the 12-MT concentration,which can be associated with meat of more intensive aroma and better taste.Keywords: Fatty aldehydes; plasmalogens; phospholipids; 12-methyltridecanal; beef; pasture; HPLC;dinitrophenylhydrazones
g/100 g fresh muscle in the muscle phospholipids of German Holstein and GermanSimmental bulls as compared with the concentrate-fed bulls. Furthermore, pasture feeding resultedin a significant increase of n-octadecanal in the muscle phospholipids of both breeds up to 39.5mg/100 g fresh muscle. The concentration of n-hexadecanal was not affected by the diet. Pasturefeeding as compared to concentrate feeding significantly decreased the concentration of n-octadec-9-enal in the muscle phospholipids. Summarizing, pasture feeding increased the 12-MT concentration,which can be associated with meat of more intensive aroma and better taste.Keywords: Fatty aldehydes; plasmalogens; phospholipids; 12-methyltridecanal; beef; pasture; HPLC;dinitrophenylhydrazones