Array-Based Sensing of Metastatic Cells and Tissues Using Nanoparticle鈥揊luorescent Protein Conjugates
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- 作者：Subinoy Rana ; Arvind K. Singla ; Avinash Bajaj ; S. Gokhan Elci ; Oscar R. Miranda ; Rubul Mout ; Bo Yan ; Frank R. Jirik ; Vincent M. Rotello
- 刊名：ACS Nano
- 出版年：2012
- 出版时间：September 25, 2012
- 年：2012
- 卷：6
- 期：9
- 页码：8233-8240
- 全文大小：458K
- 年卷期：v.6,no.9(September 25, 2012)
- ISSN：1936-086X
文摘
Rapid and sensitive methods of discriminating between healthy tissue and metastases are critical for predicting disease course and designing therapeutic strategies. We report here the use of an array of gold nanoparticle鈥揼reen fluorescent protein elements to rapidly detect metastatic cancer cells (in minutes), as well as to discriminate between organ-specific metastases and their corresponding normal tissues through their overall intracellular proteome signatures. Metastases established in a new preclinical non-small-cell lung cancer metastasis model in athymic mice were used to provide a challenging and realistic testbed for clinical cancer diagnosis. Full differentiation between the analyte cell/tissue was achieved with as little as 200 ng of intracellular protein (1000 cells) for each nanoparticle, indicating high sensitivity of this sensor array. Notably, the sensor created a distinct fingerprint pattern for the normal and metastatic tumor tissues. Moreover, this array-based approach is unbiased, precluding the requirement of a priori knowledge of the disease biomarkers. Taken together, these studies demonstrate the utility of this sensor for creating fingerprints of cells and tissues in different states and present a generalizable platform for rapid screening amenable to microbiopsy samples.