Labeling Monomeric Insulin with Renal-Clearable Luminescent Gold Nanoparticles

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• 作者：Rodrigo D. Vinluan III ; Mengxiao Yu ; Melissa Gannaway ; Justin Sullins ; Jing Xu ; Jie Zheng
• 刊名：Bioconjugate Chemistry
• 出版年：2015
• 出版时间：December 16, 2015
• 年：2015
• 卷：26
• 期：12
• 页码：2435-2441
• 全文大小：379K
• ISSN：1520-4812

文摘

In the native physiological environment, inorganic nanoparticles (NPs) often induce nonspecific protein adsorption, which could significantly alter the function of the proteins they labeled. As a result, small fluorescent dyes are still widely used in the imaging of proteins in animals due to their minimal interference with protein function. Here, we used monomeric insulin as a model and compared its bioactivity before and after labeling with renal-clearable near-infrared-emitting gold NPs. These NPs were chosen because they have high resistance to serum protein adsorption and low nonspecific accumulation. We have found that a 1:1 insulin鈥揘P ratio can be achieved, where the insulin鈥揘Ps show minimal serum protein binding with fully retained bioactivity comparable to that of unlabeled insulin. These results show a proof of concept that renal-clearable NPs can behave like small molecules in protein labeling without changing the individual protein鈥檚 function, laying down a foundation for in vivo tracking of proteins with multimodality imaging techniques.