Potentiometric Multichannel Cytometer Microchip for High-throughput Microdispersion Analysis

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• 作者：Junhoi Kim ; Eun-Geun Kim ; Sangwook Bae ; Sunghoon Kwon ; Honggu Chun
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：January 2, 2013
• 年：2013
• 卷：85
• 期：1
• 页码：362-368
• 全文大小：369K
• 年卷期：v.85,no.1(January 2, 2013)
• ISSN：1520-6882

文摘

The parallelization of microfluidic cytometry is expected to lead to considerably enhanced throughput enabling point-of-care diagnosis. In this article, the development of a microfluidic potentiometric multichannel cytometer is presented. Parallelized microfluidic channels sharing a fluid path inevitably suffer from interchannel signal crosstalk that results from electrical coupling within the microfluidic channel network. By employing three planar electrodes within a single detection channel, we electrically decoupled each channel unit, thereby enabling parallel analysis by using a single cytometer microchip with multiple microfluidic channels. The triple-electrode configuration is validated by analyzing the size and concentration of polystyrene microbeads (diameters: 1.99, 2.58, 3, and 3.68 渭m; concentration range: 2 脳 10⁵ mL^鈥? to 1 脳 10⁷ mL^鈥?) and bacterial microdispersion samples (Bacillus subtilis, concentration range: 4 脳 10⁵ CFU mL^鈥? to 3 脳 10⁶ CFU mL^鈥?). Crosstalk-free parallelized analysis is then demonstrated using a 16-channel potentiometric cytometer (maximum cross-correlation coefficients |r|: < 0.13 in all channel combinations). A detection throughput of 48鈥?00 s^鈥? was achieved; the throughout can be easily increased with the degree of parallelism of a single microchip without additional technical complexities. Therefore, this methodology should enable high-throughput and low-cost cytometry.