Genetic Manipulation for Enhancing Calcium Content in Potato Tuber

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• 作者：Sunghun Park ; Tae-Suk. Kang ; Chang-Kil. Kim ; Jeung-Sul Han ; Sunggil Kim ; Roberta H. Smith ; Leonard M. Pike ; and Kendal D. Hirschi
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2005
• 出版时间：July 13, 2005
• 年：2005
• 卷：53
• 期：14
• 页码：5598 - 5603
• 全文大小：386K
• 年卷期：v.53,no.14(July 13, 2005)
• ISSN：1520-5118

文摘

Increased calcium (Ca) in potatoes may increase the production rate by enhancing tuber quality andstorability. Additionally, increased Ca levels in important agricultural crops may help ameliorate theincidence of osteoporosis. However, the capacity to alter Ca levels in potato tubers through geneticmanipulations has not been previously addressed. Here we demonstrate that potato tubers expressingthe Arabidopsis H⁺/Ca²⁺ transporter sCAX1 (N-terminal autoinhibitory domain truncated version ofCAtion eXchanger 1) contain up to 3-fold more Ca than wild-type tubers. The increased Ca appearsto be distributed throughout the tuber. The sCAX1-expressing potatoes have normally undergonethe tuber/plant/tuber cycle for three generations; the trait appeared stable through successivegenerations. The expression of sCAX1 does not appear to alter potato growth and development.Furthermore, increased Ca levels in sCAX1-expressing tubers do not appear to alter tuber morphologyor yield. Given the preponderance of potato consumption worldwide, these transgenic plants may bea means of marginally increasing Ca intake levels in the population. To our knowledge, this studyrepresents the first attempts to use biotechnology to increase the Ca content of potatoes.Keywords: Arabidopsis; calcium; nutrition; potato; H⁺/Ca²⁺ transporter