Intracellular CO Release from Composite of Ferritin and Ruthenium Carbonyl Complexes

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• 作者：Kenta Fujita ; Yuya Tanaka ; Takeya Sho ; Shuichi Ozeki ; Satoshi Abe ; Tatsuo Hikage ; Takahiro Kuchimaru ; Shinae Kizaka-Kondoh ; Takafumi Ueno
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：December 3, 2014
• 年：2014
• 卷：136
• 期：48
• 页码：16902-16908
• 全文大小：388K
• ISSN：1520-5126

文摘

Protein cages have been utilized as templates in the development of biomaterials. Here we report protein engineering of the ferritin (Fr) cage for encapsulating carbon monoxide releasing molecules (CORMs) and release of CO gas which serves as a cell signaling molecule. The protein cages enable us to increase the half-life for CO release, providing a release rate that is 18-fold slower than the rate of a typical CORM, Ru(CO)₃Cl(glycinate) (CORM-3). Moreover, the uptake ratio of the composite is about 4-fold greater than that of CORM-3. We found that these effects enhance the activation of nuclear factor 魏B 10-fold higher than CORM-3. The protein cage of Fr thus provides the basis for new CORMs that can be used for in vitro cell research.