Toxic Identification and Evaluation of Androgen Receptor Antagonistic Activities in Acid-Treated Liver Extracts of High-Trophic Level Wild Animals from Japan

详细信息    查看全文

• 作者：Kentaro Misaki ; Go Suzuki ; Nguyen Minh Tue ; Shin Takahashi ; Masayuki Someya ; Hidetaka Takigami ; Yuko Tajima ; Tadasu K. Yamada ; Masao Amano ; Tomohiko Isobe ; Shinsuke Tanabe
• 刊名：Environmental Science & Technology
• 出版年：2015
• 出版时间：October 6, 2015
• 年：2015
• 卷：49
• 期：19
• 页码：11840-11848
• 全文大小：547K
• ISSN：1520-5851

文摘

Sulfuric acid-treated liver extracts of representative high-trophic level Japanese animals were analyzed by toxic identification and evaluation (TIE) with chemically activated luciferase expression (CALUX) and chemical analysis to elucidate androgen receptor (AR) antagonistic activities and potential contributions of organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs). The activities were detected in striped dolphins (n = 5), Stejneger鈥檚 beaked whales (n = 6), golden eagle (n = 1), and Steller鈥檚 sea eagle (n = 1) with CALUX-flutamide equivalents (FluEQs) as follow: 38 (20鈥?2), 47 (21鈥?6), 5.0, and 80 渭g FluEQ/g-lipid, respectively. The AR antagonism was detected in limited number of specimens at lower levels for finless porpoise, raccoon dog, and common cormorant. Theoretical activities (Theo-FluEQs) were calculated using the concentration of OCPs and PCBs and their IC₂₅-based relative potency (REP) values. These total contribution to CALUX-FluEQ was 126%, 84%, 53%, 55%, and 44% for striped dolphin, Steller鈥檚 sea eagle, Stejneger鈥檚 beaked whale, finless porpoise, and golden eagle, respectively, and the main contributor was p,p鈥?DDE. However, most of the activities for raccoon dog (7.6%) and common cormorant (17%) could not be explained by OCPs and PCBs. This suggests other unknown compounds could function as AR antagonists in these terrestrial species.