Gradual Immobilization Processes of Molecules during Transitions from Solute to Solid States

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• 作者：Guoliang Dai ; Gen Sazaki ; Takuro Matsui ; Katsuo Tsukamoto ; Kazuo Nakajima ; Qi Kang ; Wenrui Hu
• 刊名：Crystal Growth & Design
• 出版年：2011
• 出版时间：January 5, 2011
• 年：2011
• 卷：11
• 期：1
• 页码：88-92
• 全文大小：873K
• 年卷期：v.11,no.1(January 5, 2011)
• ISSN：1528-7505

文摘

The adsorption/desorption behavior of mobile solute molecules at a solution鈭抍rystal interface has been explored using crystals of model protein hen egg-white lysozyme (HEWL) and fluorescent-labeled HEWL (F-HEWL) molecules. We have tracked the transient processes occurring during adsorption/desorption of identical F-HEWL molecules on a tetragonal HEWL crystal surface by single-molecule visualization using a total internal reflection fluorescent microscope and pulsed discontinuous laser illumination. We found an induction period (70 min) after which the number density of F-HEWL molecules adsorbed mainly on steps increased linearly with the adsorption time. We show direct evidence that the residence time of molecules on the crystal surface gradually increases during the transition process from a solute species to the crystal after successive multistep processes.