Pinpointing Pseurotins from a Marine-Derived Aspergillus as Tools for Chemical Genetics Using a Synthetic Lethality Yeast Screen

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• 作者：Claudia M. Boot ; Nadine C. Gassner ; Jennifer E. Compton ; Karen Tenney ; Craig M. Tamble ; R. Scott Lokey ; Theodore R. Holman ; Phillip Crews
• 刊名：Journal of Natural Products
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：70
• 期：10
• 页码：1672 - 1675
• 全文大小：177K
• 年卷期：v.70,no.10(October 2007)
• ISSN：1520-6025

文摘

A new compound of mixed polyketide synthase–nonribosomal peptide synthetase (PKS/NRPS) origin, 11-O-methylpseurotin A (1), was identified from a marine-derived Aspergillus fumigatus. Bioassay-guided fractionation using a yeast halo assay with wild-type and cell cycle-related mutant strains of Saccharomyces cerevisiae resulted in the isolation of 1, which selectively inhibited a Hof1 deletion strain. Techniques including 1D and 2D NMR, HRESIMS, optical rotation, J-based analysis, and biosynthetic parallels were used in the elucidation of the planar structure and absolute configuration of 1. A related known compound, pseurotin A (2), was also isolated and found to be inactive in the yeast screen.