Highly Hydrated Deformable Polyethylene Glycol-Tethered Lipid Bilayers

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• 作者：Samira Hertrich ; Frank Stetter ; Adrian R眉hm ; Thorsten Hugel ; Bert Nickel
• 刊名：Langmuir
• 出版年：2014
• 出版时间：August 12, 2014
• 年：2014
• 卷：30
• 期：31
• 页码：9442-9447
• 全文大小：301K
• ISSN：1520-5827

文摘

The realization of a solid-supported lipid bilayer acting as a workbench for the study of membrane processes is a difficult task. For robustness, the bilayer has to be tethered to the substrate. At the same time, diffusion of the lipids and plastic deformations of the membrane should not be obstructed. Furthermore, a highly hydrated surrounding is mandatory. Here, we show that grafting of a polyethylene glycol鈥搇ipid construct (PEG2000鈥揇SPE) to a silicon oxide surface via multiple-step silane chemistry and subsequent deposition of lipids by spin-coating result in a cushioned membrane that has the desired properties. Neutron and X-ray reflectometry measurements are combined to access thickness, density, and hydration of the bilayer and the PEG cushion. We observe a spacer of 55 脜 thickness between lipid bilayer and silicon-oxide surface with a rather high hydration of up to 90 卤 3% water. While 11.5 卤 3% of the lipids are grafted to the surface, as determined from the neutron data, the diffusion constant of the lipids, as probed by diffusion of 0.5% Texas Red labeled lipids, remains rather large (D = 2.1 卤 0.1 渭m²/s), which is a reduction of only 12% compared to a supported lipid bilayer reference without immobilized lipids. Finally, AFM indentation confirms the plastic behavior of the membrane against deformation. We show that rupture of the bilayer does not occur before the deformation exceeds 40 脜. Altogether, the presented PEG-tethered lipid bilayer mimics the deformability of natural cell membranes much better than standard solid-supported lipid bilayers.