Complexation of Lysozyme with Sodium Poly(styrenesulfonate) via the Two-State and Non-Two-State Unfoldings of Lysozyme

详细信息    查看全文

• 作者：Fu-Gen Wu ; Yao-Wen Jiang ; Hai-Yuan Sun ; Jun-Jie Luo ; Zhi-Wu Yu
• 刊名：Journal of Physical Chemistry B
• 出版年：2015
• 出版时间：November 12, 2015
• 年：2015
• 卷：119
• 期：45
• 页码：14382-14392
• 全文大小：581K
• ISSN：1520-5207

文摘

To provide an in-depth understanding of the complexation mechanism of protein and polyelectrolyte, a heating鈥揷ooling鈥搑eheating protocol was employed to study the unfolding and refolding behaviors of a model protein, lysozyme, in the presence of a negatively charged polyelectrolyte, sodium poly(styrenesulfonate) (PSS). It was found that, with elevated PSS concentration, a new state (state I) was first formed via a 鈥渢wo-state鈥?conversion process and this state could further convert to a completely unfolded state (state II) via a 鈥渘on-two-state鈥?conversion. This non-two-state conversion process occurs without the coexistence of states I and II but involves the formation of various intermediate unfolded protein structures. Different from the pure lysozyme that exhibited refolding upon cooling from its heat-denatured state, lysozyme in state I could undergo unfolding upon heating but no refolding upon cooling, while lysozyme in state II did not undergo unfolding or refolding upon thermal treatments. In addition, the effects of ionic strength and molecular weight of polyelectrolyte on the unfolding and refolding behaviors of lysozyme were also investigated. The present work provides a better understanding of the principles governing protein鈥損olyelectrolyte interactions and may have implications for the fabrication of biocolloids and biofilms.