Luciferase and Luciferin Co-immobilized Mesoporous Silica Nanoparticle Materials for Intracellular Biocatalysis

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• 作者：Xiaoxing Sun ; Yannan Zhao ; Victor S.-Y. Lin ; Igor I. Slowing ; Brian G. Trewyn
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：November 23, 2011
• 年：2011
• 卷：133
• 期：46
• 页码：18554-18557
• 全文大小：802K
• 年卷期：v.133,no.46(November 23, 2011)
• ISSN：1520-5126

文摘

We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from the PEGylated exterior surface of Au-MSN to Hela cells. The effectiveness of luciferase-catalyzed luciferin oxidation and luminescence emission in the presence of intracellular ATP was measured by a luminometer. Overall, the chemical tailorability of the Au-MSN platform to retain enzyme bioactivity, the ability to codeliver enzyme and substrate, and the potential for imaging tumor growth and metastasis afforded by intracellular ATP- and glutathione-dependent bioluminescence make this platform appealing for intracellular controlled catalysis and tumor imaging.