An Automatic Analyzer for Catecholamines and Their 3-O-Methyl Metabolites Using a Micro Coulometric Flow Cell as a Postcolumn Reactor for Fluorogenic Reaction

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• 作者：Kazuko Takezawa ; Makoto Tsunoda ; Noriyuki Watanabe ; and Kazuhiro Imai
• 刊名：Analytical Chemistry
• 出版年：2000
• 出版时间：September 1, 2000
• 年：2000
• 卷：72
• 期：17
• 页码：4009 - 4014
• 全文大小：76K
• 年卷期：v.72,no.17(September 1, 2000)
• ISSN：1520-6882

文摘

A coulometric flow cell for a miniaturized LC system wasdeveloped. The cell was examined, as 3-O-methyl catecholamines were converted to their relative o-quinonesfor subsequent fluorometric and chemiluminescence detection. Its performance was evaluated in comparison withcommercially available amperometric and coulometricdetectors in terms of specification of the low dead volumeand high conversion efficiency. The fully automated small-bore LC analyzer for simultaneous determination of catecholamines and their 3-O-methyl metabolites includedprecolumn pretreatment, column switching, column separation, postcolumn oxidative conversion, fluorometricderivatization, and chemiluminescence detection. Thedetection limits were 0.3-2.0 fmol for catecholaminesand their 3-O-methyl metabolites. Because of the highsensitivity, the required volume of rat plasma sample wasonly 15 L.