Importance of Saccharomyces cerevisiae Oxidosqualene-Lanosterol Cyclase Tyrosine 707 Residue for Chair-Boat Bicyclic Ring Formation and Deprotonation Reactions
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- 作者：Tung-Kung Wu ; Tsai-Ting Wang ; Cheng-Hsiang Chang ; Yuan-Ting Liu ; Wen-Shiang Shie
- 刊名：Organic Letters
- 出版年：2008
- 出版时间：November 6, 2008
- 年：2008
- 卷：10
- 期：21
- 页码：4959-4962
- 全文大小：158K
- 年卷期：v.10,no.21(November 6, 2008)
- ISSN：1523-7052
文摘
A contact mapping strategy was applied to identify putative amino acid residues that influence the oxidosqualene-lanosterol B-ring cyclization reaction. A bicyclic intermediate with two altered deprotonation products, in conjunction with lanosterol, were isolated from the ERG7Y707X mutants, indicating that the Tyr707 residue may play a functional role in stabilizing the chair-boat bicyclic C-8 cation and the lanosteryl C-8/C-9 cation intermediates.