Gemcitabine Causes Minimal Modulation of Carboplatin-DNA Monoadduct Formation and Repair in Bladder Cancer Cells
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- 作者：Sisi Wang ; Hongyong Zhang ; Michael Malfatti ; Ralph de Vere White ; Primo N. Lara ; Jr. ; Kenneth Turteltaub ; Paul Henderson ; Chong-xian Pan
- 刊名：Chemical Research in Toxicology
- 出版年：2010
- 出版时间：November 15, 2010
- 年：2010
- 卷：23
- 期：11
- 页码：1653-1655
- 全文大小：155K
- 年卷期：v.23,no.11(November 15, 2010)
- ISSN：1520-5010
文摘
We are developing a method to identify cellular resistance to carboplatin by using accelerator mass spectrometry to measure carboplatin-DNA adducts formed from drug microdoses (1/100th the therapeutic dose). Such an approach would be particularly useful if it is still valid in combination chemotherapy. We examined whether the addition of gemcitabine, another chemotherapeutic drug, could influence carboplatin-DNA adduct levels. There were no substantial differences in the levels of carboplatin-DNA adducts in cells upon exposure to the carboplatin/gemcitabine combination at various doses and schedules. These data demonstrate that microdosing is feasible for the characterization of carboplatin resistance when given in combination with gemcitabine.