Site-Specific Radiofluorination of Biomolecules with 8-[18F]-Fluorooctanoic Acid Catalyzed by Lipoic Acid Ligase
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- 作者：Christopher R. Drake ; Natalia Sevillano ; Charles Truillet ; Charles S. Craik ; Henry F. VanBrocklin ; Michael J. Evans
- 刊名：ACS Chemical Biology
- 出版年：2016
- 出版时间：June 17, 2016
- 年：2016
- 卷：11
- 期：6
- 页码：1587-1594
- 全文大小：403K
- 年卷期：0
- ISSN：1554-8937
文摘
New methodologies for site-specifically radiolabeling proteins with 18F are required to generate high quality radiotracers for preclinical and clinical applications with positron emission tomography. Herein, we report an approach by which we use lipoic acid ligase (LplA) to conjugate [18F]-fluorooctanoic acid to an antibody fragment bearing the peptide substrate of LplA. The mild conditions of the reaction preserve antibody immunoreactivity, and the efficiency of LplA allows for >90% yield even with very small amounts of peptidic precursor (1–10 nmol). These features are advantageous compared to the current gold standard in the field. Moreover, the methodology introduces a new application for an important tool in chemical biology.