Ultrasensitive All-Carbon Photoelectrochemical Bioprobes for Zeptomole Immunosensing of Tumor Markers by an Inexpensive Visible Laser Light

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• 作者：Chengguo Hu ; Jinou Zheng ; Xiaoyang Su ; Juan Wang ; Wangze Wu ; Shengshui Hu
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：November 5, 2013
• 年：2013
• 卷：85
• 期：21
• 页码：10612-10619
• 全文大小：470K
• 年卷期：v.85,no.21(November 5, 2013)
• ISSN：1520-6882

文摘

A novel enzyme-free and all-carbon photoelectrochemical (PEC) bioprobe, based on carboxylated multiwalled carbon nanotube鈥揅ongo red鈥揻ullerene nanohybrids (MWNTCOOH鈥揅R鈥揅₆₀), for the ultrasensitive immunosensing of carcinoembryonic antigen (CEA) was reported. The MWNTCOOH鈥揅R鈥揅₆₀ nanohybrids, prepared by mechanically grinding a mixture of MWNTCOOH, C₆₀, and CR at a certain mass ratio, had good water dispersibility and high PEC conversion efficiency in visible light ranges. Covalent binding of the detection antibody of CEA on the MWNTCOOH鈥揅R鈥揅₆₀ nanohybrids produced a sensitive PEC bioprobe for detection of CEA by sandwich immunosensing. The corresponding immunosensor, employing an inexpensive and portable green laser light, possessed a wide calibration range of 1.0 pg/mL100.0 ng/mL and a low detection limit of 0.1 pg/mL (calculated 5 zmol for a 10.0 渭L sample solution) (S/N = 3), which was successfully applied to the detection of CEA in serum samples from both healthy people and cancer patients. The present work thus demonstrated the promising application of fullerene-based nanocomposites in developing highly sensitive, environmentally friendly, and cost-effective PEC biosensors.