Genetic Incorporation of 蔚-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones

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• 作者：Han Xiao ; Weimin Xuan ; Sida Shao ; Tao Liu ; Peter G. Schultz
• 刊名：ACS Chemical Biology
• 出版年：2015
• 出版时间：July 17, 2015
• 年：2015
• 卷：10
• 期：7
• 页码：1599-1603
• 全文大小：278K
• ISSN：1554-8937

文摘

Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl鈥搕RNA synthetase (PylRS)/tRNA_CUA^Pyl pair that genetically encodes the post-translationally modified amino acid, 蔚-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone mark that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.