Bioactive Surface Modification of Metal Oxides via Catechol-Bearing Modular Peptides: Multivalent-Binding, Surface Retention, and Peptide Bioactivity

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• 作者：Wen Tang ; Gina M. Policastro ; Geng Hua ; Kai Guo ; Jinjun Zhou ; Chrys Wesdemiotis ; Gary L. Doll ; Matthew L. Becker
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：November 19, 2014
• 年：2014
• 卷：136
• 期：46
• 页码：16357-16367
• 全文大小：774K
• ISSN：1520-5126

文摘

A series of multivalent dendrons containing a bioactive osteogenic growth peptide (OGP) domain and surface-binding catechol domains were obtained through solid phase synthesis, and their binding affinity to hydroxyapatite, TiO₂, ZrO₂, CeO₂, Fe₃O₄ and gold was characterized using a quartz crystal microbalance with dissipation (QCM-d). Using the distinct difference in binding affinity of the bioconjugate to the metal oxides, TiO₂-coated glass slides were selectively patterned with bioactive peptides. Cell culture studies demonstrated the bioavailability of the OGP and that OGP remained on the surface for at least 2 weeks under in vitro cell culture conditions. Bone sialoprotein (BSP) and osteocalcein (OCN) markers were upregulated 3-fold and 60-fold, respectively, relative to controls at 21 days. Similarly, 3-fold more calcium was deposited using the OGP tethered dendron compared to TiO₂. These catechol-bearing dendrons provide a fast and efficient method to functionalize a wide range of inorganic materials with bioactive peptides and have the potential to be used in coating orthopaedic implants and fixation devices.