Neucode Labels for Multiplexed, Absolute Protein Quantification
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- 作者：Gregory K. Potts ; Emily A. Voigt ; Derek J. Bailey ; Christopher M. Rose ; Michael S. Westphall ; Alexander S. Hebert ; John Yin ; Joshua J. Coon
- 刊名：Analytical Chemistry
- 出版年：2016
- 出版时间：March 15, 2016
- 年：2016
- 卷：88
- 期：6
- 页码：3295-3303
- 全文大小：520K
- ISSN：1520-6882
文摘
We describe a new method to accomplish multiplexed, absolute protein quantification in a targeted fashion. The approach draws upon the recently developed neutron encoding (NeuCode) metabolic labeling strategy and parallel reaction monitoring (PRM). Since PRM scanning relies upon high-resolution tandem mass spectra for targeted protein quantification, incorporation of multiple NeuCode labeled peptides permits high levels of multiplexing that can be accessed from high-resolution tandem mass spectra. Here we demonstrate this approach in cultured cells by monitoring a viral infection and the corresponding viral protein production over many infection time points in a single experiment. In this context the NeuCode PRM combination affords up to 30 channels of quantitative information in a single MS experiment.