Evaluation of Hyaluronan from Different Sources: Streptococcus zooepidemicus, Rooster Comb, Bovine Vitreous, and Human Umbilical Cord
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- 作者：Aviva Shiedlin ; Russell Bigelow ; William Christopher ; Saman Arbabi ; Laura Yang ; Ronald V. Maier ; Norman Wainwright ; Alice Childs ; and Robert J. Miller
- 刊名：Biomacromolecules
- 出版年：2004
- 出版时间：November 2004
- 年：2004
- 卷：5
- 期：6
- 页码：2122 - 2127
- 全文大小：173K
- 年卷期：v.5,no.6(November 2004)
- ISSN：1526-4602
文摘
Sodium hyaluronate (HA) is widely distributed in extracellular matrixes and can play a role in orchestratingcell function. Consequently, many investigators have looked at the effect of exogenous HA on cell behaviorin vitro. HA can be isolated from several sources (e.g., bacterial, rooster comb, umbilical cord) and thereforecan possess diverse impurities. This current study compares the measured impurities and the differences inbiological activity between HA preparations from these sources. It was demonstrated that nucleic acid andprotein content was highest in human umbilical cord and bovine vitreous HA and was low in bacterial androoster comb HA. Macrophages exposed to human umbilical cord HA produced significantly higher amountsof TNF-
relative to control or bacterial-derived HA. These results indicate that the source of HA shouldbe considered due to differences in the amounts and types of contaminants that could lead to widely differentbehaviors in vitro and in vivo.
relative to control or bacterial-derived HA. These results indicate that the source of HA shouldbe considered due to differences in the amounts and types of contaminants that could lead to widely differentbehaviors in vitro and in vivo.