Studying O-Linked Protein Glycosylations in Human Plasma

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• 作者：Taufika Islam Williams ; Diana A. Saggese ; David C. Muddiman
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：7
• 期：6
• 页码：2562 - 2568
• 全文大小：1454K
• 年卷期：v.7,no.6(June 2008)
• ISSN：1535-3907

文摘

Recent investigations have implicated aberrant glycosylations in various malignancies, including epithelial ovarian cancer (EOC). The protocol here identifies O-linked carbohydrate patterns in EOC plasma glycoproteins through chemical cleavage and purification of these glycans. Dialyzed plasma is subjected to reductive β-elimination with alkaline borohydride to release O-linked oligosaccharides from glycoproteins. Enrichment of released glycans, as well as removal of peptide and other contaminants, is followed by carbohydrate pattern analysis with MALDI-FT-ICR-MS.